Abstract:Objective：To investigate and analyze the current situation of dietary behaviors among middle school students in Nanjing，and to provide the basis for the implementation of middle school nutrition education. Methods：An anonymous questionnaire survey was conducted among 545 students from 5 middle schools in Nanjing by stratified cluster sampling. Results：The scores of dietary intake，healthy dietary behavior and the comprehensive score of diet were 3.20±1.11，2.08±0.86 and 5.28±1.50，respectively. Low scores on dietary intake were the consumption of eggs and milk，low scores on healthy dietary behaviors were refusing snacks and refusing drinks. There were significant differences in dietary intake in grade（F=6.508），parents’ educational level（father F=9.936，mother F=8.076）and the one-child（F=5.445，P＜0.001）. The score of healthy dietary behavior was higher in boys than in girls（t=6.162，P＜0.001）. The comprehensive scores of dietary behavior were higher in boys than in girls（t=4.171，P＜0.001），and higher in the one-child than non-one-child（t=4.561，P＜0.001）Middle school students are more enthusiastic about health attitudes in four aspects：caring for health，improving health，understanding nutrition knowledge，and enhancing dietary health，respectively 83.9%，82.6%，80.4%，80.6%，but there were only bits of students（66.4%）willing to change poor dietary habits. Junior students were more willing to change poor dietary habits than the seniors（χ2=10.958，P=0.012）. Conclusion：There were some dietary behavior problems in middle school students in Nanjing，and they had positive healthy attitudes. Middle school nutrition education should be strengthened and we should develop targeted education content according to the status quo.