Abstract:Objective：To develop and implement the standardized pretreatment process of dental reuse，reduce the risk of sharp injury，improve the cleaning effect，and ensure the quality of disinfection and sterilization. Methods：In April 2023，108 pieces of reuse devices used in the diagnosis and treatment department of stomatology of a certain grade A tertiary hospital were sent to the disinfection supply center，randomly divided into conventional manual cleaning group，conventional automatic cleaning unit and combined automatic cleaning unit according to the standardized pretreatment process，with 36 pieces for each group. Washing effect was detected by ATP biofluorescence. Results：The qualified rate of ATP biofluocence detection manual cleaning group，automatic cleaning unit，standardized pretreatment process and automatic cleaning unit are respectively 83.3%（30/36），61.1%（22/36），100%（36/36）。The RLU values in the three groups were 7.22 ± 21.32，37.61 ± 94.06，and 0 ± 0，respectively. The cleaning qualified rate of the automatic cleaning unit is significantly higher than that of the conventional manual cleaning group and the automatic cleaning unit，（χ2∶18.732 P < 0.01），with statistical significance. Conclusion：The automatic cleaning machine after the standardized pretreatment process can improve the cleaning effect and provide a basis for the standardized pretreatment process of medical reuse devices in the hospital.