Abstract:Objective：To explore the current situation of nursing culture construction in the integration process of public hospitals under the perspective of resource integration，and to provide a reference basis for hospital managers in improving and promoting nursing culture construction and other aspects. Methods：By establishing a nursing culture construction group，the hospital department strengthened nursing culture construction in terms of material culture，behavioral culture，institutional culture and spiritual culture. 1 300 nurses in acertain public hospital in Wuxi were selected for the study，and a questionnaire survey was conducted on their general information，work values and organizational culture. We also compiled statistics on the number of nurses leaving the hospital in 2021 and 2022 and the number of quarterly feedback entries on ideological dynamics and the motivation to participate in nursing activities. Results：After the practice of nursing culture construction，the total mean score of nurses’ organizational culture（90.47±18.82）and the total mean score of work values（181.83±33.50）were higher than before the implementation（P < 0.05）. The number of departures in 2022 is significantly lower than that in 2021，the number of feedback entries on thought dynamics is lower，and the motivation to participate in various activities is significantly higher. Conclusion：Nursing culture construction based on the perspective of resource integration is important to enhance the organizational culture and work values of nurses in the process of hospital integration. Helping hospitals to create a good nursing culture environment，enhance organizational cohesion.