Abstract:Objective：To understand the current score of the 16 Personality Factors Questionnaire（16PF）for talents with a master’s degree or above in the hospital. Toanalyze the differences in the scores of talents with different genders，positions and professional titles. Methods：From January to July 2021，the census will be used to include those with a master’s degree or above who have joined the workforce from 2012 to 2021 in a certain hospital，to analyze the differences in the scores of different genders，ages，professional titles，positions，etc. of the research objects. Results：A total of 300 respondents were included in the survey. According to the scale scores，females’ scores of gregariousness，sensitivity and anxiety are higher than males’，males’ assertiveness and skepticism scores are higher than females’. Medical staff’s intelligence score is higher than non-medical staff，the scores of assertiveness and daring of senior professional titles are higher than those of intermediate and junior professional titles，and the sensitivity scores are lower than those of intermediate professional titles. Conclusion：The personality factors of talents with master’s degree or above in this hospital show certain differences in different genders，positions，professional titles，etc. It is suggested that in the follow-up talent management work of the hospital，the talent management strategy should be adjusted and perfected according to the personality characteristics and career development of the talents.