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中文摘要: 目的：掌握河源地区血小板捐献者HLA Ⅰ类（-A、B位点）和HPA基因信息資料，初步建立本地区血小板基因信息库。方法：选择2017年9月6日至2020年9月6日期间在本地区捐献血小板的200例固定献血者为研究对象，采用PCR-SSP技术对其血液样本进行HLA Ⅰ类（-A、B）基因和HPA1-17系统基因分型，分析其基因频率分布情况。结果：200例样本中共检出HLA-A、B位点等位基因41种。其中HLA-A位点检出15个，HLA-B位点检出26个。HLA-A、B位点基因频率>0.05的是：A*11（0.2625）、A*2（0.2225）、A*33（0.1775）、A*24（0.1175）、A*1（0.0650）和B*58（0.1425）、B*62（0.1400）、B*46（0.1225）、B*13（0.0750）。HPA1-17系统基因分型中等位基因HPA15a、HPA3a、HPA5a、HPA2a、HPA1a呈多态性分布，其频率分别为：0.555、0.570、0.950、0.968、0.990。呈单特异性分布的等位基因是：HPA4a、HPA6a-14a，HPA16a、HPA17a，a基因频率均为1，b基因频率为0。对偶抗原不配合率前二位分别为HPA-15（37.20%）和HPA-3（37.00%）。结论：初步建立河源地区血小板捐献者HLA-A、B位点与HPA1-17系统基因信息库。
Abstract:Objective：To establish genetic database of HLA class I（-A，B locus）and HPA about platelet donors in Heyuan area. Methods：200 regular blood donors who donated platelets in the region from September 6，2017 to September 6，2020 were selected as research subjects. Polymerase chain reaction sequence specific primer（PCR-SSP）applied in genotyping for HLAclass I（-A，B locus），and HPA1-17，Analyze its gene frequency distribution. Results：Total of 41 HLA-A and B alleles were detected in 200 apheresis platelets donors. There were 15 alleles at HLA-A locus and 26 alleles at HLA-B locus. The gene frequencies of HLA-A、B> 0.05 were：A * 11（0.2625），A * 2（0.2225），A * 33（0.1775），A * 24（0.1175），A * 1（0.0650），and and HLA-B wereB * 58（0.1425），B * 62（0.1400），B * 46（0.1225），B* 13（0.0750）. The alleles with polymorphic distribution in the genotyping of HPA1-17 system were HPA15a，HPA3a，HPA5a，HPA2a and HPA1a，and their frequencies were 0.555，0.570，0.950，0.968 and 0.990 respectively. The alleles with monospecific distribution of that were HPA4a，HPA6a-14a，HPA16a and HPA17a，with Agene frequency of 1 and B gene frequency of 0. The first two incompatibility rates were HPA-15（37.20%）and HPA-3（37.00%）. Conclusion：The gene information database of HLA-A，B locus and HPA1-17 system was preliminarily established in Heyuan area .
keywords: Invalid Platelet Transfusion Machine-collected Platelets Human Platelet Alloantigen Human Leukocyte Antigen
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黄 璐,欧小懂,叶登凰,聂湘辉,刘丽华.河源地区机采血小板捐献者基因信息库的建立[J].江苏卫生事业管理,2022,33(7):976-978.
HUANG Lu,OU Xiaodong,YE Denghuang,NIE Xianghui,LIU Lihua.Establishment of Genetic Database of Platelet Donors of Mechanically Harvestedin Heyuan Area[J].Jiangsu Health System Management,2022,33(7):976-978.
黄 璐,欧小懂,叶登凰,聂湘辉,刘丽华.河源地区机采血小板捐献者基因信息库的建立[J].江苏卫生事业管理,2022,33(7):976-978.
HUANG Lu,OU Xiaodong,YE Denghuang,NIE Xianghui,LIU Lihua.Establishment of Genetic Database of Platelet Donors of Mechanically Harvestedin Heyuan Area[J].Jiangsu Health System Management,2022,33(7):976-978.
