Abstract:Objective：To investigate the burnout and psychological well-being of medical staff involved in the testing of nucleic acids during the New Coronary Pneumonia epidemic，and to provide a theoretical basis for the development of targeted psychological interventions. Methods：Using the “Questionnaire Star” mobile application software（APP），self-compiled general information questionnaire，GAD-7 Generalized Anxiety Disorder Scale，PHQ-9 Depression Screening Scale and Chinese Version of M’s Burnout Scale（Malaseh Burnout Inventory，MBI）to conduct a questionnaire survey on 58 laboratory medical staff in Suzhou who participated in the nucleic acid detection of COVID-19，and used t test and multiple linear regression model to analyze the influencing factors. Results：The three dimensions of BMI，the emotional exhaustion dimension，the depersonalization dimension，and the personal fulfillment dimension scored（25.55±11.950），（8.02±5.987），and（22.76±7.032），respectively，for a total score of（58.810 3±20.122 83）. Compared with the norm，the emotional exhaustion score was higher，and the personal achievement score was lower，and the differences were statistically significant（P < 0.05）. Univariate analysis found that anxiety，depression，age，overtime hours，health level，sleep quality，work stress，lifestress，work-life conflict，laboratory work and job burnout were statistically significant（P < 0.05）. Multiple linear regression analysis was performed on the total score of MBI，and the results showed that depression，health level，and work in the laboratory were statistically significant（P < 0.05）. Conclusion：The job burnout of medical staff during the outbreak of the pandemic is affected by depression，health level and laboratory work，suggesting that during the pandemic，we should attach significance to the mental health of laboratory medical staff，and carry out specificpsychological counseling and intervention.