Abstract:Objective：Investigate the vaccination knowledge，attitude，behavior status and knowledge needs of guardians of left-behind children and formulate targeted measures. Methods：A total of 230 guardians of left-behind children who came to our hospital for physical examination from May 2018 to October 2019 were selected as the research objects，and were divided into two groups according to the random number table method，with 115 cases in each group. The two groups of left-behind children’s guardians’ vaccination knowledge，attitudes，current status of behavior and knowledge needs were investigated and statistics，and intervention measures were implemented. The control group implemented routine interventions，and the study group strengthened interventions. The actual vaccination rate，timely delay rate and vaccination knowledge awareness rate were compared between the two groups. Results：The actual vaccination rate，timely vaccination rate，and vaccination knowledge awareness rate in the study group were higher than those in the control group（P < 0.05）. There was no significant difference in the basic awareness rate between the two groups. Conclusion：The guardians of left-behind children are not active in vaccination attitudes and behaviors. Increasing the awareness rate of vaccination among child guardians will help increase the vaccination rate and timely vaccination rate.