Abstract:Objective：To evaluate the dietary nutrition status of staffs in NanjingChildren’s Hospital by using Chinese Diet Balance Index DBI_16 and discuss the method to improve the quality of staff’s diet. Method：A questionnaire survey was conducted to investigate the dietary intake and the nutritional knowledge（with FFQ method）of the staff in hospital. The dietary quality was evaluated by Chinese Diet Balance Index DBI_16 and the relationship between the nutritional knowledge score and the dietary quality was analyzed. Results：The LBS score of the staffs of the Children’s Hospital is 14（10，19）. About 56.3% of the staffs were mild，and 5.1% were moderate or severe under intake of foods. The HBS score of the staffs of the Children’s Hospital is 3（1，6）. 17.5% of the staffs were mild over intake of foods，and 5.1% were moderate or severe over intake of foods. The DQDscore of the staffs of the Children’s Hospital is18（15，22}.75.7% of the staffs were mild dietary unbalance，and 2.6% were moderate or severe dietary unbalance. There was insufficient intake of milk and fish and shrimp，and excessive intake of poultry and livestock. The staff had less types of food. There are statistical differences in DBI scores between genders. The dietary quality of women was better than men. The staff’s nutritional knowledge awareness score is 2（1，3）. The higher the nutritional knowledge score of the staff，the higher the quality of their diet. Conclusion：The dietary nutritional status of the staffs in this hospital were not good，and there was a slight dietary unbalance. It is necessary to strengthen the dietary nutrition education to improve the dietary quality of the staff.