Abstract:Objective：To analyze the current situation of nosocomial infection in certaingrade-A tertiary hospital from 2018 to 2019，understand the development trend of nosocomial infection in the hospital，and explore the strategies for prevention and control of nosocomial infection，so as to reduce the occurrence of nosocomial infection. Methods：A retrospective analysis of the inpatients and nosocomial infection cases was performed. Results：There was no significant difference in the incidence of nosocomial infection between different genders. The nosocomial infection rate showed an upward trend with the growth of age（P < 0.001）. The hospital infection rate of inpatients in different seasons had no significant difference.71.52% of the patients with nosocomial infection were distributed in the department of internal medicine.lower respiratory tract infection（33.66%）was the main cause. Antibiotics，ventilator，central venous catheterization and urinary catheterization are the risk factors of nosocomial infection. Conclusion：It is necessary to strengthen the monitoring of key groups，key links and key parts of nosocomial infection，reasonably use antibacterial drugs，reduce invasive operation and strengthen the training of hospital infection knowledge. Through the implementation of half a year，the hospital infection control in the second half of 2020 has achieved initial results.